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Myosin heavy-chain mRNA is present in both myofibrillar and subsarcolemmal regions of muscle fibres.

ABSTRACT: Hybridization in situ with riboprobes to the myosin heavy-chain slow isoform showed that, in the rat soleus muscle, the myosin heavy-chain mRNA was distributed throughout the myofibres. There was greater density of autoradiographic grains in the subsarcolemmal regions of the fibres, but there was also a considerable number of grains in the core myofibrillar region of the fibres. Microdensitometry showed that the grain density in the myofibrillar region was approximately half that in the subsarcolemmal rim; this would correspond to some 70% of the mRNA being present in the myofibrillar region. The results are consistent with the hypothesis that myosin is synthesized on polyribosomes present in the intermyofibrillar cytoplasm.

SUBMITTER: Hesketh J

PROVIDER: S-EPMC1151581 | biostudies-other | 1991 Oct

REPOSITORIES: biostudies-other

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