A glucuronyltransferase involved in glucuronoxylan synthesis in pea (Pisum sativum) epicotyls.
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ABSTRACT: A particulate enzyme preparation made from epicotyls of 1-week-old etiolated pea (Pisum sativum) seedlings was shown to incorporate glucuronic acid from UDP-D-[U-14C]glucuronic acid into a hemicellulosic polysaccharide. Optimum conditions for the incorporation include the presence of Mn2+ ions at between 4 and 10 mmol/litre and a pH between 5 and 6. UDP-D-xylose at 1 mmol/litre allows incorporation to continue for at least 8 h. In its absence, the reaction stops within 30 min. Analysis of the product by partial and total acid hydrolysis, followed by paper chromatography or electrophoresis, indicates that the polysaccharide produced is a glucuronoxylan.
SUBMITTER: Waldron KW
PROVIDER: S-EPMC1152097 | biostudies-other | 1983 Jul
REPOSITORIES: biostudies-other
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