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Importance of sites of tracer administration and blood sampling in relation to leucine metabolism. Practical considerations.


ABSTRACT: For the same infusion site of L-[1-14C]leucine, sampling downstream of arterial blood underestimates leucine turnover, whereas sampling of venous blood overestimates turnover. Further, the lungs release a small but consistent amount of leucine into the blood. Unlabelled leucine also is produced by the portal-drained viscera, and some is removed immediately by the liver. These sources of leucine should thus be considered in turnover calculations.

SUBMITTER: Pell JM 

PROVIDER: S-EPMC1152348 | biostudies-other | 1983 Sep

REPOSITORIES: biostudies-other

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