Unknown

Dataset Information

0

Changes in the DNA content of amoebae of Dictyostelium discoideum during growth and development.


ABSTRACT: A fluorimetric assay has been used to determine the DNA content of amoebae of Dictyostelium discoideum during growth and development. Amoebae grown in axenic culture tended to be multinucleate and had a greater DNA content than amoebae grown with a bacterial substrate, which were mononucleate. During the first 10 h of development there was little change in the DNA content of amoebae grown with a bacterial substrate, but the average DNA content per cell in amoebae grown axenically decreased as the amoebae became virtually mononucleate. Amoebae at 10 h development that had been harvested during exponential axenic growth were divided into two populations by countercurrent distribution in a polymer two-phase system. DNA content indicated that one population was largely in the G2-phase of the cell cycle, whereas the other population was largely in the G1-phase. Similar results were obtained at 10 h development with amoebae harvested during the stationary phase of axenic growth, although these amoebae start development all in the G2-phase of the cell cycle. Spores had a low DNA content, indicating that they were in G1-phase. It is proposed that all amoebae in G2-phase after early development differentiate, after mitosis, into spores and that stalk cells are formed from amoebae that remain in G1-phase after 10 h development.

SUBMITTER: Sharpe PT 

PROVIDER: S-EPMC1153289 | biostudies-other | 1984 Feb

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC6326426 | biostudies-literature
| S-EPMC3188802 | biostudies-literature
| S-EPMC7029077 | biostudies-literature
| S-EPMC8163159 | biostudies-literature
| S-EPMC4627915 | biostudies-literature
| S-EPMC4172474 | biostudies-literature
2010-06-09 | E-TABM-803 | biostudies-arrayexpress
| S-EPMC4094278 | biostudies-literature
| S-EPMC3743828 | biostudies-literature
| S-EPMC3293572 | biostudies-literature