Unknown

Dataset Information

0

The ability of adenosine to decrease the concentration of fructose 2,6-bisphosphate in isolated hepatocytes. A cyclic AMP-mediated effect.


ABSTRACT: The presence of adenosine (25-250 microM) or of 2-chloroadenosine (2.5-100 microM) in the incubation medium caused a marked decrease in the concentration of fructose 2,6-bisphosphate in isolated hepatocytes. This effect was accompanied by an increase in the concentration of cyclic AMP, an activation of phosphorylase and of fructose 2,6-bisphosphatase, and an inactivation of pyruvate kinase and of 6-phosphofructo-2-kinase. As a rule, the changes in the fructose 2,6-bisphosphate-modifying system were slower but more persistent than those in the activities of phosphorylase and pyruvate kinase. The effect of the nucleoside to decrease the concentration of fructose 2,6-bisphosphate was not affected by an inhibitor of adenosine transport and could not be obtained in a liver high-speed supernatant. These data indicate that the effect of adenosine to decrease the concentration of fructose 2,6-bisphosphate is mediated by the stimulation of adenylate cyclase, secondary to the binding of adenosine to membranous receptors. Like glucagon, 2-chloroadenosine stimulated gluconeogenesis in isolated hepatocytes, whereas adenosine had an opposite effect.

SUBMITTER: Bartrons R 

PROVIDER: S-EPMC1153319 | biostudies-other | 1984 Feb

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1152320 | biostudies-other
| S-EPMC3961670 | biostudies-literature
| S-EPMC165633 | biostudies-literature
| S-EPMC1144606 | biostudies-other
| S-EPMC1144159 | biostudies-other
| S-EPMC1147977 | biostudies-other
| S-EPMC1149955 | biostudies-other
| S-EPMC1158592 | biostudies-other
| S-EPMC4196155 | biostudies-literature
| S-EPMC1135658 | biostudies-other