A problem in the radiochemical assay of glucose-6-phosphatase in muscle.
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ABSTRACT: A radiochemical assay for glucose-6-phosphatase, which depends on the absorption of radiolabelled substrate on to a nascent precipitate of BaSO4 and Zn(OH)2, can give rise to erroneously high activities. Adsorption on to a Fe(OH)3 precipitate does not expose the incubation medium to extreme alkaline conditions and provides a satisfactory and simple assay. This method demonstrates a very low activity of the enzyme in mammalian muscle, in contrast with previous findings.
SUBMITTER: Lackner R
PROVIDER: S-EPMC1153387 | biostudies-other | 1984 Mar
REPOSITORIES: biostudies-other
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