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Large-scale isolation of human erythrocyte Ca2+-transport ATPase.


ABSTRACT: A rapid procedure for preparing large quantities of purified erythrocyte Ca2+-transport ATPase is presented. The method involves: (1) fast preparation of calmodulin-deficient, essentially haemoglobin-free, erythrocyte membranes by molecular filtration using Pellicon filters; (2) solubilization of membrane proteins by deoxycholate; and (3) a batch procedure using calmodulin-Sepharose 4B gel for purification of Ca2+-transport ATPase.

SUBMITTER: Gietzen K 

PROVIDER: S-EPMC1153836 | biostudies-other | 1982 Oct

REPOSITORIES: biostudies-other

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