Underestimation of metabolic rates owing to reincorporation of 14CO2 in the perfused rat liver.

ABSTRACT: (14)CO(2) production by perfused rat livers was simulated by infusing NaH(14)CO(3) into the perfusate. Recovery of label as (14)CO(2) gas + perfusate bicarbonate was 45-85%. Rates of (14)CO(2) exchange in the liver are 3-70 times greater than net rates of CO(2) production. Therefore (14)CO(2) reincorporation can lead to significant underestimations of rates of oxidation of (14)C-labelled substrates in liver.

SUBMITTER: Tomera JF 

PROVIDER: S-EPMC1153950 | biostudies-other | 1982 Oct

REPOSITORIES: biostudies-other