Crayfish abdominal muscle adenylate cyclase. Studies on the stimulation by a Ca2+-binding protein.
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ABSTRACT: A plasma-membrane preparation of crayfish muscle showed an adenylate cyclase activity which is inhibited to about 80% of its original activity by 100 microM-EGTA. Measurements of the enzyme activity in the presence of 100 microM-EGTA and various concentrations of Ca2+ revealed an increase in enzyme activity of about 400%, indicating an adenylate cyclase which is dependent on Ca2+ for activity. Fluphenazine (1 mM), a blocker of the Ca2+-binding protein calmodulin, decreased enzyme activity to zero. The enzyme can be re-activated by the addition of certain concentrations of calmodulin to the assay medium. This suggests that crayfish muscle adenylate cyclase is dependent on Ca2+ and calmodulin for activity.
SUBMITTER: Sedlmeier D
PROVIDER: S-EPMC1154362 | biostudies-other | 1983 May
REPOSITORIES: biostudies-other
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