Unknown

Dataset Information

0

Monoclonal antibodies that immunoreact with a cation-stimulated plant membrane ATPase.


ABSTRACT: Hybridoma technology has been used successfully to generate monoclonal-antibody probes against protoplast membrane antigens. Hybridomas secreting monoclonal antibodies that either inhibit or stimulate a putative plasma-membrane marker enzyme, (K+ + Mg2+)-stimulated pH 6.5 ATPase, have been identified and cloned. The specificity of monoclonal-antibody probes on the activity of other phosphate-hydrolysing enzymes has also been examined. The production and identification of monospecific antibodies capable of immunoreacting with particular component proteins in a complex plant membrane mixture highlight the usefulness of hybridoma methodology for the enzymologist, especially since such monoclonal antibodies can be used in the purification of proteins by immunoaffinity techniques.

SUBMITTER: Chin JJ 

PROVIDER: S-EPMC1158191 | biostudies-other | 1982 Apr

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC7349930 | biostudies-literature
| S-EPMC3733320 | biostudies-literature
| S-EPMC5597267 | biostudies-literature
| S-EPMC6978629 | biostudies-literature
| S-EPMC6981118 | biostudies-literature
| S-EPMC9045258 | biostudies-literature
| S-EPMC4878533 | biostudies-literature
| S-EPMC11002603 | biostudies-literature
| S-EPMC10536087 | biostudies-literature
| S-EPMC5654892 | biostudies-literature