Unknown

Dataset Information

0

Monosaccharide transport into lactating-rat mammary acini.


ABSTRACT: The uptake and release of 3-O-methyl-D-[3H]glucose at 37 degrees C by acini, prepared from lactating-rat mammary gland with collagenase, was inhibited by glucose, phloretin, cytochalasin B, HgCl2 and low temperature. Uptake and phosphorylation of 2-deoxy-D-[3H]glucose, studied in greater detail, could be ascribed to a specific, saturable, inhibitable, process of apparent Km 16 mM and Vmax. approx. 56 nmol/min per mg of protein, plus a non-specific, non-inhibitable process that was monitored with [14C]fructose. The mean rate of uptake of 5 mM-2-deoxyglucose (16 nmol/min per mg of protein) was similar to the rate of consumption of 5 mM-glucose, suggesting that transport was a rate-limiting step in the overall metabolism of glucose. This accords with evidence for a glucose gradient across the plasma membrane.

SUBMITTER: Threadgold LC 

PROVIDER: S-EPMC1158377 | biostudies-other | 1982 May

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1153326 | biostudies-other
| S-EPMC1144772 | biostudies-other
| S-EPMC3408423 | biostudies-literature
| S-EPMC1164962 | biostudies-other
| S-EPMC1187507 | biostudies-other
| S-EPMC1144413 | biostudies-other
| S-EPMC1162139 | biostudies-other
| S-EPMC1166237 | biostudies-other
2017-08-20 | GSE60539 | GEO
| S-EPMC8575188 | biostudies-literature