Fractionation of membrane proteins on phenylboronic acid-agarose.
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ABSTRACT: Detergent-solubilized plasma-membrane proteins from lymphocytes of patients with chronic lymphocytic leukaemia were applied to phenylboronic acid-agarose. Some of the polypeptides were bound specifically and subsequently eluted with sorbitol. Immunoprecipitation analyses showed that no surface immunoglobulin M was bound, but that most of the histocompatibility antigens HLA-A, HLA-B, HLA-C and HLA-DR were.
SUBMITTER: Williams GT
PROVIDER: S-EPMC1158459 | biostudies-other | 1982 Jul
REPOSITORIES: biostudies-other
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