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Uptake and degradation of mast-cell granules by mouse peritoneal macrophages.


ABSTRACT: 35S-labelled mast-cell granules isolated from mouse mastocytomas were added to mouse macrophages in vitro. The granules were avidly phagocytosed, and subsequently the radioactivity was released to the medium as inorganic [35S]sulphate. After pulse-labelling, a total of about 80% of the cell-associated radioactivity was thus released in the course of 24 h, indicating an extensive breakdown of the sulphated polysaccharides, mainly heparin, present in the granules. The uptake of the mast-cell granules caused pronounced, but reversible, spreading of the macrophages.

SUBMITTER: Lindahl U 

PROVIDER: S-EPMC1161248 | biostudies-other | 1979 Jul

REPOSITORIES: biostudies-other

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