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Biosynthesis and turnover of outer-membrane proteins in Escherichia coli ML308-225.


ABSTRACT: Isolated outer membranes and outer-membrane extracts from Escherichia coli ML308-225 in the early-exponential growth phase contain more protein than do corresponding preparations from late-exponential- or stationary-phase bacteria. Isotope-dilution experiments show that this is due to a loss of protein from the membrane during the exponential growth phase. Inhibition of bacterial growth and protein synthesis stabilizes the outer-membrane-protein concentration. Protein synthesis in the absence of bacterial growth results in higher concentrations of protein in the outer membrane.

SUBMITTER: Allen RJ 

PROVIDER: S-EPMC1161321 | biostudies-other | 1979 Aug

REPOSITORIES: biostudies-other

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