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Absolute configuration of dolichol.


ABSTRACT: A derivative of dolichol was formed and then chemically degraded to a small fragment containing the sole centre of asymmetry of the original molecule. Polarimetric comparison of this derivative with a standard prepared from (R)-citronellol showed dolichol to have an S-configuration at C-3. To determine the optical purity of dolichol a diastereoisomeric derivative was prepared and compared with standard diastereoisomers, which could be resolved by high-pressure liquid chromatography. Dolichols from pig liver, human liver and hen oviduct were analysed by this procedure and were all found to be greater than 95% S-configuration.

SUBMITTER: Adair WL 

PROVIDER: S-EPMC1162022 | biostudies-other | 1980 Sep

REPOSITORIES: biostudies-other

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