Lack of deviation from Michaelis--Menten kinetics for pig heart fumarase.
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ABSTRACT: Studies of steady-state kinetics of fumarase in the usual substrate-concentration range from 0.1 Km to 10 Km and in the high substrate-concentration range from 10 Km to 200 Km are described. The purpose is to investigate reports of substrate inhibition and oscillatory kinetics. In the normal substrate-concentration range, no deviations from hyperbolic kinetics were found, and in the extended concentration range, up to more than 200 times the Km value, no substrate inhibition was demonstrated. A discussion of the discrepancies between the mentioned reports of deviations from the hyperbolic kinetics and the present findings is given.
SUBMITTER: Andersen B
PROVIDER: S-EPMC1162048 | biostudies-other | 1980 Sep
REPOSITORIES: biostudies-other
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