Unknown

Dataset Information

0

Isomyosins in human type 1 and type 2 skeletal muscle fibres.


ABSTRACT: Human myosin from different skeletal muscles was analysed in a non-denaturing gel system, and the isoenzyme composition correlated with the histochemical composition of the muscle. Two components (SM1 and SM2) were associated with type 1 (slow-twitch) fibres, and three (FM1, FM2 and FM3) with type 2 (fast-twitch) fibres. Light-chain analysis was performed in sodium dodecyl sulphate/polyacrylamide gels. There are three light chains (LCs1a, LCS1b and LCs2) in type 1 fibres, and three (LCf1, LCf2 and LCf3) in type 2 fibres. LCf1 and LCs1b co-migrate in sodium dodecyl sulphate gels. The ratio of LCf3/LCf2 is correlated with the distribution of the individual fast isoenzymes. These results explain apparent discrepancies in the literature concerning the light-chain distribution of human myosin.

SUBMITTER: Fitzsimons RB 

PROVIDER: S-EPMC1162594 | biostudies-other | 1981 Jan

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC3350594 | biostudies-literature
| S-EPMC9825974 | biostudies-literature
| S-EPMC4633712 | biostudies-literature
| S-EPMC9931674 | biostudies-literature
| S-EPMC2045119 | biostudies-literature
| S-EPMC7663642 | biostudies-literature
| S-EPMC3609830 | biostudies-literature
| S-EPMC1148981 | biostudies-other
| S-EPMC1153856 | biostudies-other
| S-EPMC3287155 | biostudies-literature