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Effects of dicyclohexylcarbodi-imide on proton translocation coupled to fumarate reduction in anaerobically grown cells of Escherichia coli K-12.

ABSTRACT: The addition of dicyclohexylcarbodi-imide to anaerobic cells of Escherichia coli K12 decreases both the observed extent of proton translocation coupled to fumarate reduction by endogenous substrates and the t 1/2 of proton re-entry after such translocation, but does not affect fumarate uptake. Dicyclohexylcarbodi-imide also inhibits fumarate reductase activity in cell extracts.

SUBMITTER: Gutowski SJ

PROVIDER: S-EPMC1164300 | biostudies-other | 1976 Dec

REPOSITORIES: biostudies-other

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Proton translocation coupled to electron flow from endogenous substrates to fumarate in anaerobically grown Escherichia coli K12.

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