Unknown

Dataset Information

0

Biospecific-elution chromatography with 'imphilytes' as stationary phases.

ABSTRACT: Six out of seven enzymes tested (four of them nicotinamide nucleotide-dependent dehydrogenases) showed differences in chromatographic behaviour in the presence and absence of their biospecific ligands, when chromatographed on immobilized amphipathic ampholytes ('imphilytes') as stationary phases. Some enzymes were adsorbed more tightly, others less tightly, in the presence of ligands. These results have implications for enzyme purification in general, and for some types of affinity chromatography in particular.

SUBMITTER: Yon RJ 

PROVIDER: S-EPMC1164500 | biostudies-other | 1977 Feb

REPOSITORIES: biostudies-other

Json Xml

Similar Datasets

Unknown Chiral Stationary Phases for Liquid Chromatography: Recent Developments.
| S-EPMC6429359 | biostudies-literature
Unknown Modelling of Hydrophilic Interaction Liquid Chromatography Stationary Phases Using Chemometric Approaches.
| S-EPMC5746734 | biostudies-literature
Unknown Highly stabilized, polymer-lipid membranes prepared on silica microparticles as stationary phases for capillary chromatography.
| S-EPMC4706447 | biostudies-literature
Unknown Tandem dye-ligand chromatography and biospecific elution applied to the purification of glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides.
| S-EPMC1154102 | biostudies-other
Unknown A Comparative Study of Advanced Stationary Phases for Fast Liquid Chromatography Separation of Synthetic Food Colorants.
| S-EPMC6321072 | biostudies-literature
Unknown Application of Homochiral Alkylated Organic Cages as Chiral Stationary Phases for Molecular Separations by Capillary Gas Chromatography.
| S-EPMC6274383 | biostudies-literature
Unknown Comparative Chiral Separation of Thalidomide Class of Drugs Using Polysaccharide-Type Stationary Phases with Emphasis on Elution Order and Hysteresis in Polar Organic Mode.
| S-EPMC8746373 | biostudies-literature
Unknown Retention behavior of alkyl-substituted polycyclic aromatic sulfur heterocycle isomers in gas chromatography on stationary phases of different selectivity.
| S-EPMC5497302 | biostudies-literature
Unknown Enantioseparation of 5,5'-Dibromo-2,2'-Dichloro-3-Selanyl-4,4'-Bipyridines on Polysaccharide-Based Chiral Stationary Phases: Exploring Chalcogen Bonds in Liquid-Phase Chromatography.
| S-EPMC7794968 | biostudies-literature
Unknown Novel MIPs-Parabens based SPE Stationary Phases Characterization and Application.
| S-EPMC6767171 | biostudies-literature