Unknown

Dataset Information

0

Studies on the cell-free biosynthesis of beta-lactam antibiotics.


ABSTRACT: Cell walls of Cephalosporium acremonium mycelia were lysed by enzyme preparations from either Helix pomatia (snail) digestive juice or Cytophaga. The yield of protoplasts depended on the lytic-enzyme preparation and the age of the culture, and it increased after the mycelia were pretreated with dithiothreitol. A cell-free preparation, obtained by osmotic lysis of protoplasts, synthesized labelled penicillin N from L-[14C]valine. Approx. 0.03-0.06% of the amino acid was incorporated into penicillin N. Under conditions of penicillin N synthesis, the broken-protoplast preparation failed to produce significant amounts of cephalosporin C or its precursors, deacetylcephalosporin C and deacetoxycephalosporin C.

SUBMITTER: Bost PE 

PROVIDER: S-EPMC1164653 | biostudies-other | 1977 Mar

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC3525771 | biostudies-literature
| S-EPMC7229998 | biostudies-literature
| S-EPMC8480522 | biostudies-literature
| S-EPMC3209169 | biostudies-literature
| S-EPMC4258230 | biostudies-literature
| S-EPMC5913952 | biostudies-literature
| S-EPMC3123045 | biostudies-literature
| S-EPMC8456660 | biostudies-literature
| S-EPMC4972527 | biostudies-literature
| S-EPMC1538687 | biostudies-literature