Unknown

Dataset Information

0

Characterization of a plasmin-digest fragment of rabbit immunoglobulin gamma that binds antigen and complement.


ABSTRACT: Rabbit immunoglobulin gamma (IgG) was digested with plasmin after being left for 15 min at pH2.5, 30 degrees C followed by a rapid increase in the pH to 7. The fragment antigen and complement binding (Facb) was isolated and characterized chemically and biologically. Sequence studies showed that the C-terminal quarter of the heavy chain had been removed, the split occurring at a lysine-alanine bond in the sequence Thr-Ile-Ser-Lys-Ala-Arg. The fragment Facb retained the capacity to precipitate with antigen and the precipitate caused activation of the first component of complement of the same order as that of acid-treated IgG. Both Facb and acid-treated IgG showed a fall in complement fixation relative to the native molecule of 30-40%.

SUBMITTER: Colomb M 

PROVIDER: S-EPMC1165206 | biostudies-other | 1975 Feb

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC5522293 | biostudies-literature
| S-EPMC4076286 | biostudies-literature
| S-EPMC1270632 | biostudies-other
| S-EPMC1183712 | biostudies-other
| S-EPMC1162460 | biostudies-other
| S-EPMC5459932 | biostudies-literature
| S-EPMC1177145 | biostudies-other
| S-EPMC3048689 | biostudies-literature
| S-EPMC4979565 | biostudies-literature
| S-EPMC5508854 | biostudies-other