Subcellular localization of pulmonary antiotensin-converting enzyme (kininase II).
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ABSTRACT: Goat antibodies to pig lung angiotensin-converting enzyme (kininase II) were conjugated to microperoxidase. Rat lung tissue, previously incubated with non-immune goat serum, was incubated with the antibody-microperoxidase conjugate and then with H2O2 and 3,3-diaminobenzidine. Electron microscopy revealed reaction product on the plasma membrane and caveolae of endothelial cells, especially those of capillaries and venules. These results support the hypothesis that angiotensin I and bradykinin are metabolized by enzymes on the luminal surface of pulmonary endothelial cells.
SUBMITTER: Ryan JW
PROVIDER: S-EPMC1165329 | biostudies-other | 1975 Feb
REPOSITORIES: biostudies-other
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