Ligand binding to ferrocytochrome c at high pH.
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ABSTRACT: Ferrocytochrome c has been shown to bind two molecules of CO at pH 14. The second CO is thought to be bound only when the cytochrome c molecule is denatured, and once bound appears to be spectrally silent. Insolubilization of native cytochrome c prevents the binding of the second CO molecule. A scheme is proposed to explain these observations based on evidence from static titrations and flash-photolysis experiments, use of carboxymethyl cytochrome c and insoluble cytochrome c, and use of cyanide instead of CO as a ligand.
SUBMITTER: Moore TA
PROVIDER: S-EPMC1165447 | biostudies-other | 1975 May
REPOSITORIES: biostudies-other
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