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Kinetic studies on the hydroxylation of p-coumaric acid to caffeic acid by spinach-beet phenolase.


ABSTRACT: 1. A spectrophotometric assay is described that enables the hydroxylation of p-coumaric acid to caffeic acid, catalysed by spinach-beet phenolase, to be followed continuously. 2. Initial-velocity and inhibitor studies indicate that the order of substrate addition is oxygen, p-coumaric acid and electron donor, with an irreversible step separating the binding of each substrate. 3. Caffeic acid is most likely to act as electron donor at the active site; other electron donors, such as ascorbic acid, NADH and dimethyltetrahydropteridine, function mainly to recycle cofactor amounts of caffeic acid. 4. A reaction scheme, consistent with these data, is proposed.

SUBMITTER: McIntyre RJ 

PROVIDER: S-EPMC1165638 | biostudies-other | 1975 Aug

REPOSITORIES: biostudies-other

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