The molecular-weight distribution of glycosaminoglycans.
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ABSTRACT: 1. A rapid and sensitive method for the accurate estimation of the molecular-weight distribution of keratan sulphate and chondroitin sulphate isolated from adult bovine nasal septum and intervertebral disc is described. The method utilizes gel chromatography of reductively labelled glycosaminoglycan and end-group estimation of number-average molecular weight for each fraction across the peak of eluted glycosaminoglycan. 2. Chain-length distribution data obtained by this procedure are used to evaluate mechanisms of chondroitin sulphate biosynthesis.
SUBMITTER: Hopwood JJ
PROVIDER: S-EPMC1165877 | biostudies-other | 1973 Dec
REPOSITORIES: biostudies-other
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