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The amino acid sequence around the reactive cysteine residue in human plasma Factor XII.

ABSTRACT: 1. Activated human plasma factor XIII was 65% inhibited by iodo[(14)C]acetate and incorporated 0.6 mol of label into the alpha subunit, which eventually was allowed to form a precipitate. 2. All the label was recovered as S-carboxymethylcysteine in a tetra-peptide of sequence Gly-Gln-Cys-Trp.

SUBMITTER: Holbrook JJ

PROVIDER: S-EPMC1165912 | biostudies-other | 1973 Dec

REPOSITORIES: biostudies-other

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Amino acid sequences around the cysteine residue of calf lens -crystallin.

Project description:1. Calf lens alpha-crystallin was carboxymethylated with radioactive sodium iodoacetate to label the thiol group. 2. The protein was then digested with trypsin or alternatively fractionated in urea to obtain the acidic (A) chains, which were then digested with trypsin. Either procedure gave two radioactive peptides containing carboxymethylcysteine. 3. These two peptides were closely related: the longer form contained 28 amino acid residues, and the shorter lacked two residues at the N-terminal end of the longer form. 4. The amino acid sequence of the peptides have been determined. 5. No evidence for the presence of more than one cysteine residue/chain was found. 6. The question of the molecular weight of the chains is discussed.

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Bovine pepsinogens and pepsins. The sequence around a reactive aspartyl residue.

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The amino acid sequence around the active-site cysteine and histidine residues of stem bromelain.

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Amino acid sequence around the thiol and reactive acyl groups of human complement component C4.

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Amino acid sequence around the active-site serine residue in the acyltransferase domain of goat mammary fatty acid synthetase.

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