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Role of mixed-function amine oxidases in N-hydroxylation of 2-acetamidofluorene by hamster liver microsomal preparations.

ABSTRACT: Pretreatment of hamsters with 3-methylcholanthrene (100mg/kg body wt.) 24h before death did not appreciably change the extent of N-oxide formation when hepatic microsomal preparations were incubated with NN-dimethylaniline as substrate. In contrast, the N-hydroxylation of 2-acetamidofluorene was increased severalfold in hepatic microsomal preparations from pretreated animals. Under these conditions there were no appreciable changes in cytochrome P-450 content and NADPH-cytochrome c reductase activity. On the basis of these comparative data, it is suggested that amine oxidase is not involved in N-hydroxylation of 2-acetamidofluorene.

SUBMITTER: Lotlikar PD

PROVIDER: S-EPMC1166069 | biostudies-other | 1973 Dec

REPOSITORIES: biostudies-other

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