Project description:Typical multiomics studies employ separate methods for DNA, RNA, and protein sample preparation, which is labor intensive, costly, and prone to sampling bias. We describe a method for preparing high-quality, sequencing-ready DNA and RNA, and either intact proteins or mass-spectrometry-ready peptides for whole proteome analysis from a single sample. This method utilizes a reversible protein tagging scheme to covalently link all proteins in a lysate to a bead-based matrix and nucleic acid precipitation and selective solubilization to yield separate pools of protein and nucleic acids. We demonstrate the utility of this method to compare the genomes, transcriptomes, and proteomes of four triple-negative breast cancer cell lines with different degrees of malignancy. These data show the involvement of both RNA and associated proteins, and protein-only dependent pathways that distinguish these cell lines. We also demonstrate the utility of this multiomics workflow for tissue analysis using mouse brain, liver, and lung tissue.

2024-03-08 | GSE241433 | GEO

One-pot method for preparing DNA, RNA, and protein for multiomics analysis I

2024-03-08 | GSE241432 | GEO

Characterization of transcripts bound by LARP1 in the free 40S fraction isolated by sucrose gradients

Project description:RNA binding protein LARP1 binds preferentially to mRNAs containing a 5' Tract of Oligo Pyrimidines (5'-TOP) motif

2017-11-30 | GSE99502 | GEO