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Studies of the polysaccharide fraction from the lipopolysaccharide of Pseudomonas alcaligenes.

ABSTRACT: Studies of the lipopolysaccharide of Pseudomonas alcaligenes strain BR 1/2 were extended to the polysaccharide moiety. The crude polysaccharide, obtained by mild acid hydrolysis of the lipopolysaccharide, was fractionated by gel filtration. The major fraction was the phosphorylated polysaccharide, for which the approximate proportions of residues were; glucose (2), rhamnose (0.7), heptose (2-3), galactosamine (1), alanine (1), 3-deoxy-2-octulonic acid (1), phosphorus (5-6). The heptose was l-glycero-d-manno-heptose. The minor fractions from gel filtration contained free 3-deoxy-2-octulonic acid, P(i) and PP(i). The purified polysaccharide was studied by periodate oxidation, methylation analysis, partial hydrolysis, and dephosphorylation. All the rhamnose and part of the glucose and heptose occur as non-reducing terminal residues. Other glucose residues are 3-substituted, and most heptose residues are esterified with condensed phosphate residues, possibly in the C-4 position. Free heptose and a heptosylglucose were isolated from a partial hydrolysate of the polysaccharide. The location of galactosamine in the polysaccharide was not established, but either the C-3 or C-4 position appears to be substituted and a linkage to alanine was indicated. In its composition, the polysaccharide from Ps. alcaligenes resembles core polysaccharides from other pseudomonads: no possible side-chain polysaccharide was detected.

SUBMITTER: Lomax JA

PROVIDER: S-EPMC1166327 | biostudies-other | 1974 Jun

REPOSITORIES: biostudies-other

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Studies of polysaccharide fractions from the lipopolysaccharide of Pseudomonas aeruginosa N.C.T.C. 1999.

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| S-EPMC1165596 | biostudies-literature

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Functional characterization of WaaL, a ligase associated with linking O-antigen polysaccharide to the core of Pseudomonas aeruginosa lipopolysaccharide.

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Pseudomonas alcaligenes

Project description:Pseudomonas alcaligenes overview

| PRJNA171776 | ENA

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Project description:Pseudomonas alcaligenes Raw sequence reads

| PRJNA1032269 | ENA

Further studies of the chemical composition of the lipopolysaccharide of Pseudomonas aeruginosa.

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| S-EPMC1178387 | biostudies-other

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