Unknown

Dataset Information

0

The alkali-labile linkage between keratan sulphate and protein.


ABSTRACT: Keratan sulphate was isolated from adult intervertebral disc in 90% yield by sequential digestion of the whole tissue with papain, Pronase and Proteus vulgaris chondroitin sulphate lyase. Treatment of this preparation with alkali cleaved a glycosidic bond between N-acetylgalactosamine and threonine and produced, by an alkali-catalysed ;peeling' reaction, an unsaturated derivative of N-acetylgalactosamine which reacted as a chromogen in the Morgan-Elson reaction, but remained covalently bonded to the keratan sulphate chain. This derivative was reduced and labelled by alkaline NaB(3)H(4). The substituent at position 3 of N-acetylgalactosamine in the keratan sulphate-protein linkage was identified as a disaccharide, N-acetylneuraminylgalactose, which was isolated from the reaction mixture after alkali treatment.

SUBMITTER: Hopwood JJ 

PROVIDER: S-EPMC1168049 | biostudies-other | 1974 Jul

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1131530 | biostudies-other
| S-EPMC1150043 | biostudies-other
| S-EPMC1151414 | biostudies-literature
| S-EPMC1130918 | biostudies-literature
| S-EPMC1183737 | biostudies-other
| S-EPMC1168106 | biostudies-other
| S-EPMC3779837 | biostudies-literature
| S-EPMC1158229 | biostudies-other
| S-EPMC1146928 | biostudies-other
| S-EPMC1173756 | biostudies-other