Unknown

Dataset Information

0

The kinetics of coupled enzyme reactions. Applications to the assay of glucokinase, with glucose 6-phosphate dehydrogenase as coupling enzyme.


ABSTRACT: 1. A theory is developed to account for the kinetics of coupled-enzyme reactions without assuming that the second reaction follows first-order kinetics. 2. A simple procedure is described for applying the theory to the practical design of enzyme assays. 3. The validity of the theory is confirmed for the assay of glucokinase with glucose 6-phosphate dehydrogenase as coupling enzyme. 4. The possibility of extending the theory to three or more coupled reactions is discussed.

SUBMITTER: Storer AC 

PROVIDER: S-EPMC1168067 | biostudies-other | 1974 Jul

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC3154929 | biostudies-literature
| S-EPMC7679969 | biostudies-literature
| S-EPMC6792049 | biostudies-literature
| S-EPMC1153552 | biostudies-other
| S-EPMC5624969 | biostudies-literature
| S-EPMC4475474 | biostudies-literature
| S-EPMC7595223 | biostudies-literature
| S-EPMC2879355 | biostudies-literature
| S-EPMC7905371 | biostudies-literature
| S-EPMC1214693 | biostudies-literature