Unknown

Dataset Information

0

Glycosyltransferases in the Golgi membranes of onion stem.


ABSTRACT: Cell fractions consisting largely of Golgi membranes were prepared from the meristematic region of the onion. Several enzyme activities were found to be localized in these fractions: inosine diphosphatase, galactosyltransferases and glucosyltransferases. The fractions catalysed the transfer of [(14)C]galactose from UDP-galactose to endogenous and cell-sap acceptors, to N-acetylglucosamine and to ovalbumin. In the presence of bovine alpha-lactalbumin, transfer to glucose (lactose synthesis) was catalysed. [(14)C]Glucose was transferred from UDP-glucose to endogenous and cell-sap acceptors, to cellobiose and to fructose (sucrose synthesis). All these activities were latent, being potentiated by detergents (Triton X-100 or sodium deoxycholate). The characteristics of some of these enzyme activities are described and their biological significance is discussed.

SUBMITTER: Powell JT 

PROVIDER: S-EPMC1168270 | biostudies-other | 1974 Aug

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC2707253 | biostudies-literature
| S-EPMC8616560 | biostudies-literature
| S-EPMC6130340 | biostudies-literature
| S-EPMC2795549 | biostudies-literature
| S-EPMC2132995 | biostudies-literature
| S-EPMC3641799 | biostudies-other
| S-SCDT-10_15252-EMBJ_2022111869 | biostudies-other
| S-EPMC1083864 | biostudies-literature
| S-EPMC1847662 | biostudies-literature
2014-04-14 | PXD000557 | Pride