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Transport of catecholamines by resealed chromaffin-grnaule "ghosts".

ABSTRACT: A method is described for the preparation of resealed chromaffin-granule ;ghosts'. The lysis and rapid purification procedures provide ;ghosts' in approximately 70% yield from crude granules; the preparation contains 0.1mumol of catecholamine/mg of protein (as compared with 2.8mumol/mg in unlysed granules), of which about one third is inside the ;ghosts'. The ;ghosts' retain their ability to accumulate catecholamines, a process dependent on Mg-ATP and inhibited by reserpine, and a simple assay for this transport is described.

SUBMITTER: Phillips JH 

PROVIDER: S-EPMC1168498 | biostudies-other | 1974 Nov

REPOSITORIES: biostudies-other

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