Ontology highlight
ABSTRACT:
SUBMITTER: van de Locht A
PROVIDER: S-EPMC1169916 | biostudies-other | 1997 Jun
REPOSITORIES: biostudies-other
van de Locht A A Bode W W Huber R R Le Bonniec B F BF Stone S R SR Esmon C T CT Stubbs M T MT
The EMBO journal 19970601 11
Previous crystal structures of thrombin indicate that the 60-insertion loop is a rigid moiety that partially occludes the active site, suggesting that this structural feature plays a decisive role in restricting thrombin's specificity. This restricted specificity is typified by the experimental observation that thrombin is not inhibited by micromolar concentrations of basic pancreatic trypsin inhibitor (BPTI). Surprisingly, a single atom mutation in thrombin (E192Q) results in a 10(-8) M affinit ...[more]