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Structure and function of an archaeal topoisomerase VI subunit with homology to the meiotic recombination factor Spo11.


ABSTRACT: In all organisms, type II DNA topoisomerases are essential for untangling chromosomal DNA. We have determined the structure of the DNA-binding core of the Methanococcus jannaschii DNA topoisomerase VI A subunit at 2.0 A resolution. The overall structure of this subunit is unique, demonstrating that archaeal type II enzymes are distinct from other type II topoisomerases. However, the core structure contains a pair of domains that are also found in type IA and classic type II topoisomerases. Together, these regions may form the basis of a DNA cleavage mechanism shared among these enzymes. The core A subunit is a dimer that contains a deep groove that spans both protomers. The dimer architecture suggests that DNA is bound in the groove, across the A subunit interface, and that the two monomers separate during DNA transport. The A subunit of topoisomerase VI is homologous to the meiotic recombination factor, Spo11, and this structure can serve as a template for probing Spo11 function in eukaryotes.

SUBMITTER: Nichols MD 

PROVIDER: S-EPMC1171681 | biostudies-other | 1999 Nov

REPOSITORIES: biostudies-other

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Structure and function of an archaeal topoisomerase VI subunit with homology to the meiotic recombination factor Spo11.

Nichols M D MD   DeAngelis K K   Keck J L JL   Berger J M JM  

The EMBO journal 19991101 21


In all organisms, type II DNA topoisomerases are essential for untangling chromosomal DNA. We have determined the structure of the DNA-binding core of the Methanococcus jannaschii DNA topoisomerase VI A subunit at 2.0 A resolution. The overall structure of this subunit is unique, demonstrating that archaeal type II enzymes are distinct from other type II topoisomerases. However, the core structure contains a pair of domains that are also found in type IA and classic type II topoisomerases. Toget  ...[more]

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