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Conformational analysis of deoxyribonucleic acid from PM2 bacteriophage. The effect of size on supercoil shape.


ABSTRACT: Laser light-scattering studies of bacteriophage PM2 DNA showed the molecule to have mol.wt. 5.9 X 10(6) and root-mean -square radius 125 nm at an ionic strength of 0.2 mol/litre. Computer-generated curves compatible with these data were compared with the experimental interference curve for several structural models of the molecules. The data fit best to an asymmetric four-armed planar molecule in which all four arms emerge from or close to the one area of the molecule. This contrasts with the smaller DNA molecules investigated, which have shown a three-armed molecule, whose symmetry varies with primary structure.

SUBMITTER: Campbell AM 

PROVIDER: S-EPMC1172806 | biostudies-other | 1976 Apr

REPOSITORIES: biostudies-other

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