Unknown

Dataset Information

0

Covalent chromatography. Preparation of fully active papain from dried papaya latex.


ABSTRACT: 1. A Sepharose-(glutathione-2-pyridyl disulphide) conjugate has been prepared. 2. Its use in a new type of chromatography, covalent chromatography by thiol-disulphide interchange, is described. 3. With this technique, papain containing 1 intact catalytic site [thiol with high reactivity towards 2,2'-dipyridyl disulphide (2-Py-S-S-2-Py) at pH4] per mol of protein is readily prepared both from dried papaya latex and from commercial 2xcrystallized partially active papain. 4. The catalysis of the hydrolysis of alpha-N-benzoyl-l-arginine ethyl ester at pH6.0, 25.0 degrees C, I=0.3 by fully active papain thus prepared is characterized by K(m)=18.2+/-<0.1mm and k(cat.)=16.4+/-0.5s(-1).

SUBMITTER: Brocklehurst K 

PROVIDER: S-EPMC1177736 | biostudies-other | 1973 Jul

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1138849 | biostudies-other
| S-EPMC1164109 | biostudies-other
| S-EPMC1153538 | biostudies-other
| S-EPMC1163189 | biostudies-other
| S-EPMC1186404 | biostudies-other
| S-EPMC5756445 | biostudies-literature
| S-EPMC8720089 | biostudies-literature
| S-EPMC2225369 | biostudies-literature
| S-EPMC7701290 | biostudies-literature
| S-EPMC3381983 | biostudies-literature