The N-acetylhexosaminidase components of the ram testis and epididymis.

ABSTRACT: Three and four N-acetylhexosaminidase components, from ram testis and epididymis respectively, have been separated by ion-exchange chromatography on DEAE-cellulose. Although they all have the same molecular weight (approx. 140000) and very similar catalytic properties towards the synthetic substrates, 4-methylumbelliferyl N-acetyl-beta-glucosaminide and N-acetyl-beta-galactosaminide, isoelectric focusing of the individual components showed that each had a distinct pI value. Isoelectric focusing has also been used to demonstrate the occurrence of multiple forms in ejaculated ram semen.

SUBMITTER: Bullock S 

PROVIDER: S-EPMC1177738 | biostudies-other | 1973 Jul

REPOSITORIES: biostudies-other