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Preparation and characterization of antigen and antibody adsorbents covalently coupled to an inorganic carrier.


ABSTRACT: Immunoadsorbents were prepared by the use of an inorganic carrier. The proteins were covalently coupled to the carrier through a silane coupling agent. An immunoadsorbent prepared with human gamma-globulin was found to complex with specific antibodies. These antibodies could be recovered in high yield and purity from the adsorbent. Similarly, insolubilized antibodies to l-asparaginase were used to isolate and purify the enzyme from crude extracts. These insolubilized antibodies are extremely stable with time and may be reused many times.

SUBMITTER: Weetall HH 

PROVIDER: S-EPMC1178857 | biostudies-other | 1970 Apr

REPOSITORIES: biostudies-other

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