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Affinity chromatography of thiol-containing purines and ribonucleic acid.

ABSTRACT: Evidence is presented for the specific interaction of 6-mercaptopurine with mercurated cellulose. Following from this, a new method is described for the affinity chromatography of thiol-containing molecules and of RNA containing incorporated 6-thioguanosine on columns of mercurated cellulose. This technique may find application in the study of RNA metabolism and gene expression.

SUBMITTER: Melvin WT 

PROVIDER: S-EPMC1183813 | biostudies-other | 1977 Dec

REPOSITORIES: biostudies-other

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