Unknown

Dataset Information

0

Substrate specificity and kinetic properties of alpha-galactosidases from Vicia faba.


ABSTRACT: 1. The hydrolysis of a variety of galactosides and other glycosides by alpha-galactosidases I and II of Vicia faba was studied. 2. The effect of temperature on kinetic parameters was also examined. 3. Both enzymes are inhibited by excess of substrate (p-nitrophenyl alpha-d-galactoside); with enzyme I this is competitive and is caused by the galactosyl moiety. 4. Enzyme I is inhibited by oligosaccharides possessing terminal non-reducing galactose residues and to a smaller extent by l-arabinose and d-fucose. 5. The effect of pH on K(m) and V(max.) values suggests that carboxyl and imidazole groups are involved in the catalytic activity of enzyme I. 6. Photo-oxidation experiments with enzyme I also suggest that an imidazole group is present at the active site.

SUBMITTER: Dey PM 

PROVIDER: S-EPMC1185067 | biostudies-other | 1969 Oct

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1184603 | biostudies-other
| S-EPMC5429004 | biostudies-literature
| PRJNA679419 | ENA
| PRJNA81415 | ENA
| PRJNA400521 | ENA
| PRJNA1025910 | ENA
| PRJNA774948 | ENA
| PRJNA994676 | ENA
| PRJNA227061 | ENA
| PRJNA259999 | ENA