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Analysis of the visible spectra of some sperm-whale ferrimyoglobin derivatives.


ABSTRACT: The visible spectra of sperm-whale metmyoglobin and its fluoride, hydroxide, cyanide, azide, cyanate, formate, acetate, thiocyanate and nitrite derivatives were measured and resolved into Gaussian components. A linear correlation between the intensity of the band at 18500cm.(-1) and the magnetic susceptibility is found, suggesting that the band is the beta-band of the low-spin form. The analysed intensity of this band can be used to determine the relative proportions of the two spin states present. The spectra of the pure high-spin and low-spin components of each derivative can be reconstructed. Correlations between the energy of the near-infrared band and the visible bands of the high-spin form, and between the position and intensity of the 16000cm.(-1) band, are demonstrated. The relationship between the position of the Soret band and the magnetic susceptibility can be explained in terms of the equilibrium between spin states.

SUBMITTER: Smith DW 

PROVIDER: S-EPMC1187209 | biostudies-other | 1968 Nov

REPOSITORIES: biostudies-other

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