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Purification of an acid alpha-glucosidase by dextran-gel filtration.

ABSTRACT: 1. The behaviour of rat liver alpha-glucosidases on dextran gel (Sephadex G-100) columns was studied. A ;retardation' of an acid alpha-glucosidase activity was observed. This activity was identified as lysosome alpha-(1-->4)-glucosidase. A single gel-filtration step resulted in a 700-fold purification of the enzyme. The same technique was also used to purify the acid alpha-glucosidase of human kidney. 2. The acid alpha-glucosidases of both tissues show very similar pH optima when tested with maltose or glycogen as substrate.

SUBMITTER: Auricchio F

PROVIDER: S-EPMC1198270 | biostudies-other | 1967 Oct

REPOSITORIES: biostudies-other

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Further purification and characterization of the acid alpha-glucosidase.

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