Unknown

Dataset Information

0

Organic pyrophosphates as substrates for human alkaline phosphatases.


ABSTRACT: 1. Purified human liver and small-intestinal alkaline orthophosphatases release inorganic phosphate at appreciable rates from a variety of organic pyrophosphate substrates. 2. The pyrophosphatase action is inhibited by Mg(2+) ions at concentrations that activate the hydrolysis of orthophosphate substrates by these enzymes. 3. The results of mixed-substrate experiments, denaturation studies with heat or urea and starch-gel electrophoresis suggest that both orthophosphatase and pyrophosphatase activities are, in each preparation, properties of a single enzyme. 4. Intestinal phosphatase shows greater pyrophosphatase activity relative to orthophosphatase than the liver enzyme.

SUBMITTER: Eaton RH 

PROVIDER: S-EPMC1198456 | biostudies-other | 1967 Dec

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1198689 | biostudies-other
| S-EPMC1161148 | biostudies-other
| S-EPMC2795515 | biostudies-literature
| S-EPMC1187603 | biostudies-other
| S-EPMC7055307 | biostudies-literature
| S-EPMC4303301 | biostudies-literature
| S-EPMC3593980 | biostudies-literature
| S-EPMC7261903 | biostudies-literature
| S-EPMC4548154 | biostudies-literature
| S-EPMC1153246 | biostudies-other