Unknown

Dataset Information

0

Polyamines and nucleic acid metabolism in chick embryo. Incorporation of labelled precursors into nucleic acids of subcellular fractions and polyribosomal patterns.


ABSTRACT: 1. An increase in polyamine concentration, caused by inhibiting the amine oxidase activities with iproniazid, increased the incorporation of [(3)H]orotic acid into chick-embryo RNA and DNA. On the other hand, a decrease in polyamine concentration, obtained by causing an increase in amine oxidase activities, decreased [(3)H]orotic acid incorporation into nucleic acids. This was particularly evident for nuclear DNA and ribosomal RNA. 2. Polyribosomal patterns obtained by sucrose-density-gradient centrifugation showed highest radioactivity in the regions of 259s and 280s aggregates in those embryos in which the polyamine contents were enhanced, whereas a decrease in the radioactivity was observed when the polyamine concentrations were decreased. 3. The activity of DNA-dependent RNA polymerase, assayed in the same experimental conditions, also varied in the same fashion with changes in polyamine concentration.

SUBMITTER: Moruzzi G 

PROVIDER: S-EPMC1198712 | biostudies-other | 1968 May

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1264546 | biostudies-other
| S-EPMC1265111 | biostudies-other
| S-EPMC1185211 | biostudies-other
| S-EPMC2267454 | biostudies-literature
| S-EPMC6751280 | biostudies-literature
| S-EPMC1148060 | biostudies-other
| S-EPMC1264658 | biostudies-other
| S-EPMC5598472 | biostudies-literature
| S-EPMC7058932 | biostudies-literature
| S-EPMC4847719 | biostudies-literature