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A novel method for observing proteins in vivo using a small fluorescent label and multiphoton imaging.


ABSTRACT: A novel method for the fluorescence detection of proteins in cells is described in the present study. Proteins are labelled by the selective biosynthetic incorporation of 5-hydroxytryptophan and the label is detected via selective two-photon excitation of the hydroxyindole and detection of its fluorescence emission at 340 nm. The method is demonstrated in this paper with images of a labelled protein in yeast cells.

SUBMITTER: Botchway SW 

PROVIDER: S-EPMC1199672 | biostudies-other | 2005 Sep

REPOSITORIES: biostudies-other

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