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High-efficiency liquid-scintillation counting of 14C-labelled material in aqueous solution and determination of specific activity of labelled proteins.


ABSTRACT: 1. Inexpensive scintillation mixtures are described which enable the detection of as little as 40mumuc of (14)C in aqueous solution with an efficiency of counting of over 80%. 2. A rapid method for the counting of alkaline, acidic and neutral aqueous solutions of up to 1ml. volume is described. Ethanol or 2-ethoxyethanol is used as blending agent. 3. The scintillation counting of alkaline solutions is applied to the accurate determination of the specific activity of (14)C-labelled proteins from plant tissues. 4. Attention has been paid to the importance of a standardized washing procedure for the removal of all traces of radioactive material from glassware.

SUBMITTER: Hall TC 

PROVIDER: S-EPMC1207196 | biostudies-other | 1965 Sep

REPOSITORIES: biostudies-other

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