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THE SEPARATION OF FERRITIN AND HAEMOSIDERIN FOR STUDIES IN THE METABOLISM OF IRON.


ABSTRACT: 1. Ferritin and haemosiderin in diluted tissue cannot be quantitatively separated by centrifugation unless the pH is brought below 6. 2. These iron-storage substances can be separated from one another and from haemoglobin by ion-exchange chromatography on CM-cellulose, when good recoveries of total tissue iron are obtained. 3. The ferritin fraction has been identified by its solubility, its appearance under the electron microscope, crystallization with Cd(2+) and the fact that it corresponds quantitatively to the heat-stable portion of the tissue non-haem iron.

SUBMITTER: DRYSDALE JW 

PROVIDER: S-EPMC1215205 | biostudies-other | 1965 Apr

REPOSITORIES: biostudies-other

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