Unknown

Dataset Information

0

Enzyme-to-enzyme channelling of Krebs cycle metabolic intermediates in Caco-2 cells exposed to [2-13c]propionate.


ABSTRACT: The generation of 13C-labelled lactate by colon carcinoma cells of the Caco-2 line incubated for 120 min in the presence of [2-13C]propionate (10 mM) was assessed by 13C NMR. About 10% of the total amount of 13C-labelled lactate was recovered in the cell pellet and displayed a [2-13C]lactate/[3-13C]lactate isotopomer ratio of 1.18 +/- 0.01. An even higher isotopomer ratio of 1.53 +/- 0.14 was observed in the case of 13C-labelled lactate released by the cells into the incubation medium. These findings indicate that, in the Caco-2 cells, metabolic intermediates of the Krebs cycle undergo enzyme-to-enzyme channelling in the sequence of reactions catalysed by succinyl-CoA synthetase, succinate dehydrogenase and fumarase.

SUBMITTER: Malaisse WJ 

PROVIDER: S-EPMC1217564 | biostudies-other | 1996 Aug

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC7073566 | biostudies-literature
| S-EPMC5976638 | biostudies-literature
| S-EPMC10472834 | biostudies-literature
| S-EPMC5223029 | biostudies-literature
| S-EPMC5747319 | biostudies-literature
| S-EPMC1479758 | biostudies-other
| S-EPMC8455617 | biostudies-literature
| S-EPMC4346564 | biostudies-literature
| S-EPMC4666537 | biostudies-literature
| S-EPMC6400043 | biostudies-literature