Interaction of Ras with phosphoinositide 3-kinase gamma.
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ABSTRACT: Phosphoinositide 3-kinase gamma (PI3Kgamma) can be activated in vitro by both alpha and betagamma subunits of heterotrimeric G-proteins and does not interact with p85, the regulatory subunit of PI3Kalpha. Here we demonstrate the binding of Ras to PI3Kgamma in vitro. An N-terminal region of PI3Kgamma was identified as a binding site for Ras. After co-expression with PI3Kgamma in COS-7 cells, Ras induced only a modest increase in PI3K activity compared with the stimulation of PI3Kalpha by Ras in the same cells.
SUBMITTER: Rubio I
PROVIDER: S-EPMC1218747 | biostudies-other | 1997 Sep
REPOSITORIES: biostudies-other
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